Coding
fuGFP-link

Part:BBa_K4488012:Design

Designed by: Donovan Wu, Jackie Yau, Oliver Nicholls, Jasmin Li   Group: iGEM22_Sydney_Australia   (2022-09-30)

Fusion of free-use GFP with CBDcex (cellulose-binding domain) at the C-terminal end with a linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The construct has a constitutive RBS (BBa_B0034). A 45bp linker (BBa_K4488006 ) was inserted between fuGFP and CBDcenA. A 6bp cloning scar (“accgcc”) was also inserted downstream of the linker which is found in BBa_K1321341.

Source

The design of the part was based on our previous construct BBa_K4488008 . The fuGFP sequence can be found here (BBa_K3814004).

References